top of page
(AO)013  20x flat.tif

PulmoScience Lab
Leiden

Our research

0B9A1041 klein.jpg

ADVANCED
CELL CULTURE

  • ALI-PxEC

  • Organoids

  • Lung-Chips

  • hiPSC

UNADJUSTEDNONRAW_thumb_2015.jpg

REPAIR &
REGENERATION

  • Epithelial Dysfunction

  • Epithelial - Immune cross-talk

  • Epithelial - Endothelial cross-talk

  • AATD

0B9A1115 klein.jpg

HOST
DEFENCE

  • Host - Bacterial interactions

  • Host - Viral interactions

  • Antimicrobial Peptides

Our Cell Culture

Techniques

0B9A1115 klein.jpg

Insert cultures are the most used cell culture platform in our laboratory. We culture primary nasal, tracheal, bronchial and alveolar epithelial cells alone, or in combination with other cells and culture these at the air-liquid interface.

In our research we use feeder-free primary nasal, bronchial and alveolar  organoid cultures. We use organoids for expansion and propagation of epithelial cells and studies on development and repair.

0B9A1001 klein.jpg

In our laboratory we use the commercial Organ-Chip platform from Emulate Inc. for Airway and Alveolar cultures to study the effects of breathing-like stresses on cell behavior  and have developed an in-house vascularized Alveolus Lung-Chip to study cellular cross-talk in lung injury.

hiPSC colonies 100x.tif

Human induced pluripotent stem cell (hiPSC)-derived alveolar and endothelial cells are used in our lab to study repair and disease pathogenesis.

OUR LAB

2024-10 No Students cropped.jpg

PulmoScience Lab Leiden

​

Respiratory Cell Biology and Immunology is a fascinating area of research. It is not only essential for our understanding of the pathogenesis of (chronic) lung diseases, but also for the prevention, and treatment of these diseases.

​

Research by the PulmoScience Lab is conducted in a clinical department where basic, translational and clinical science meet. Research is mainly focused on tissue repair and host defences in various lung diseases.

 

Epithelial cells that line the airway and the alveoli take center stage in our research. Understanding the function of these cells in health and disease is essential for understanding their role in chronic inflammation, repair of injured lung tissue, and infections.

OUR HEAD

Anne M. van der Does, PhD

Anne van der Does is Associate Professor, and head of the PulmoScience Lab, the research lab of the Department of Pulmonology of the Leiden University Medical Center (LUMC).

Her research activities involve the development of advanced lung cell culture models and use of these models in research focused on repair & regeneration of lung tissue and respiratory host defense. She chairs the Lung-on-Chip theme group of the Dutch Organ-on-Chip consortium (hDMT), is actively involved in the European and Dutch Respiratory Society and chairs the International Antimicrobial Host Defence Peptide Network (iAMPNet).
Beyond research, she is involved in teaching (bio)medical students, supervising PhD-students and postdocs, and teaching activities outside the LUMC. She furthermore actively promotes animal-free research innovations through public engagements.

​​

​Dr. van der Does received her Ph.D. from the Department of Infectious Diseases at the LUMC before completing a 4-year postdoc at the Karolinska Institutet, in Stockholm, Sweden after which she continued her career in the PulmoScience Lab of the LUMC.

Anne vd Does.jpg

"I am passionate about contributing to science through the development of advanced cell culture models. By applying these innovative systems in my research on lung diseases, I aim to contribute to animal-free biomedical innovation." 

Contact Us

Do you have a question, or want to inquire about an internship?

Thank you for submitting!

Address

Albinusdreef 2, 2300 RC, Leiden, the Netherlands

Building 1, Floor D3

Follow us

  • Linkedin

Vacancies

No vacancies

  • Original_edited
bottom of page