We use primary nasal epithelial cell (PNEC) cultures for studies on how farm dust may protect against asthma development and to study SARS-CoV-2 infection biology.
We use primary tracheal epithelial cell (PTEC) cultures to investigate host-virus interactions, including SARS-CoV-2 infection biology.
We use primary bronchial epithelial cell (PBEC) cultures to study host-microbe interactions and responses to airborne exposures such as cigarette smoke. We furthermore study airway epithelial biology and train researchers in these cultures.
We use primary alveolar epithelial cell cultures to study repair and regeneration in the context of lung damage and to study Mycobacterium tuberculosis and SARS-CoV-2 infection biology.
We use feeder-free airway epithelial organoid cultures to study interactions of the airway epithelium with Mycobacterium tuberculosis, radiation biology and regeneration & repair.
We use feeder-free organoid cultures to expand alveolar type-2 epithelial cells for culture in Transwell or Lung-Chips. Organoids are additionally used to study (repair of) the alveolar micro-environment, alone or in co-culture with endothelial or structural cells.
We use organoid technology to establish patient-derived NSCLC tumor cultures that display a high preservation of the characteristics of the original tumor.
Our Airway Lung-Chip model is used to study how breathing biomechanics such as airflow and dynamic stretch affect primary airway epithelial cell biology .
We use the Alveolus Lung-Chip to study how endothelial cells contribute to alveolar repair and investigate effects of dynamic stretch on primary alveolar cell biology.
We use the Alveolus Lung-Chip as a starting point to develop a Fibrosis Lung-Chip model to study COVID-19-related lung damage.
Alveolar type-2 cells
We use hiPSC to generate alveolar type-2 cells. We aim to improve the differentiation protocols and use the cells to study alveolar repair. We also use these cells to develop environmental and genetic models of alveolar damage.
We are using hiPSC-derived endothelial cells to investigate how a microenvironment shapes the lung microvascular endothelial cell phenotype, and how these contribute to alveolar repair.