
Advanced
Cell Culture

ALI-PxEC
ALI-PNEC
We use primary nasal epithelial cell (PNEC) cultures for studies on how farm dust may protect against asthma development and to develop a co-culture with commensal bacteria
​
​





ALI-PxEC
ALI-PTEC
We use primary tracheal epithelial cell (PTEC) cultures to investigate host-virus interactions, including SARS-CoV-2 infection biology.
​
​
​





ALI-PxEC
ALI-PBEC
We use primary bronchial epithelial cell (PBEC) cultures to study host-microbe interactions and responses to airborne exposures such as cigarette smoke. We furthermore study airway epithelial biology and train researchers in these cultures.





ALI-PxEC
ALI-PAEC
We use primary alveolar epithelial cell cultures to study repair and regeneration in the context of lung damage and to study Mycobacterium tuberculosis and SARS-CoV-2 infection biology.
​


_edited.jpg)


Organoids
Airway Lung-Organoids
We use feeder-free airway epithelial organoid cultures to study interactions of the airway epithelium with Mycobacterium tuberculosis, radiation biology and regeneration & repair.
​
​





Organoids
Alveolus Lung-Organoids
We use feeder-free organoid cultures to expand alveolar type-2 epithelial cells for culture in Transwell or Lung-Chips. Organoids are additionally used to study (repair of) the alveolar micro-environment, alone or in co-culture with endothelial or structural cells.





Lung-Chips
Airway Lung-Chip
Our Airway Lung-Chip model is used to study how breathing biomechanics such as airflow and dynamic stretch affect primary airway epithelial cell biology .
​





Lung-Chips
Alveolus Lung-Chip
We use a commercial Organs-on-Chips platform from Emulate Inc, to study how dynamic stretch affects alveolar epithelial cell biology.
​
​



_edited.jpg)

Lung-Chips
Alveolus Lung-Chip
An In-House Alveolus-on-Chip is used to study the interaction between 3D vasculature (vascularized hydrogel) and the epithelial compartment in the context of COPD.
​


_edited.jpg)


Lung-Chips
Fibrosis Lung-Chip
We use the Alveolus Lung-Chip as a starting point to develop a Fibrosis Lung-Chip model to study COVID-19-related lung damage.
​
​
​





hiPSC
Alveolar type-2 cells
We use hiPSC to generate alveolar type-2 cells. We aim to improve the differentiation protocols and use the cells to study alveolar repair. We also use these cells to develop environmental and genetic models of alveolar damage.


_edited.jpg)


hiPSC
Endothelial cells
We are using hiPSC-derived endothelial cells to investigate how a microenvironment shapes the lung microvascular endothelial cell phenotype​, and how these contribute to alveolar repair​.
​
_edited.jpg)


