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Cell Culture

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ALI-PxEC

ALI-PNEC

We use primary nasal epithelial cell (PNEC) cultures for studies on how farm dust may protect against asthma development and to develop a co-culture with commensal bacteria

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ALI-PxEC

ALI-PTEC

We use primary tracheal epithelial cell  (PTEC) cultures to investigate host-virus interactions, including SARS-CoV-2 infection biology.

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ALI-PxEC

ALI-PBEC

We use primary bronchial epithelial cell (PBEC) cultures to study host-microbe interactions and responses to airborne exposures such as cigarette smoke. We furthermore study airway epithelial biology and train researchers in these cultures.

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ALI-PxEC

ALI-PAEC

We use primary alveolar epithelial cell cultures to study repair and regeneration in the context of lung damage and to study Mycobacterium tuberculosis and SARS-CoV-2 infection biology.

Annemarie
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Organoids

Airway Lung-Organoids

We use feeder-free airway epithelial  organoid cultures to study interactions of the airway epithelium with Mycobacterium tuberculosis, radiation biology and regeneration & repair.

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Organoids

Alveolus Lung-Organoids

We use feeder-free organoid cultures to expand alveolar type-2 epithelial cells for culture in Transwell or Lung-Chips. Organoids are additionally used to study (repair of) the alveolar micro-environment, alone or in co-culture with endothelial or structural cells.

Annemarie
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Lung-Chips

Airway Lung-Chip

Our Airway Lung-Chip model is used to study how breathing biomechanics such as airflow and dynamic stretch affect primary airway epithelial cell biology .

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Annemarie
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Lung-Chips

Alveolus Lung-Chip

We use a commercial Organs-on-Chips platform from Emulate Inc, to study how dynamic stretch affects  alveolar epithelial  cell biology.

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Annemarie
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Lung-Chips

Alveolus Lung-Chip

An In-House Alveolus-on-Chip is used to study the interaction between 3D vasculature (vascularized hydrogel) and the epithelial compartment in the context of COPD.

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Annemarie
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Lung-Chips

Fibrosis Lung-Chip

We use the Alveolus Lung-Chip as a starting point to develop a Fibrosis Lung-Chip model to study COVID-19-related lung damage.

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Annemarie
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hiPSC

Alveolar type-2 cells

We use hiPSC to generate alveolar type-2 cells. We aim to improve the differentiation protocols and use the cells to study alveolar repair. We also use these cells to develop environmental and genetic models of alveolar damage.

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hiPSC

Endothelial cells

We are using hiPSC-derived endothelial cells to investigate how a microenvironment shapes the lung microvascular endothelial cell phenotype​, and how these contribute to alveolar repair​.

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