Advanced
Cell Culture
ALI-PxEC
ALI-PNEC
We use primary nasal epithelial cell (PNEC) cultures for studies on how farm dust may protect against asthma development and to develop a co-culture with commensal bacteria
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ALI-PxEC
ALI-PTEC
We use primary tracheal epithelial cell (PTEC) cultures to investigate host-virus interactions, including SARS-CoV-2 infection biology.
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ALI-PxEC
ALI-PBEC
We use primary bronchial epithelial cell (PBEC) cultures to study host-microbe interactions and responses to airborne exposures such as cigarette smoke. We furthermore study airway epithelial biology and train researchers in these cultures.
ALI-PxEC
ALI-PAEC
We use primary alveolar epithelial cell cultures to study repair and regeneration in the context of lung damage and to study Mycobacterium tuberculosis and SARS-CoV-2 infection biology.
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Organoids
Airway Lung-Organoids
We use feeder-free airway epithelial organoid cultures to study interactions of the airway epithelium with Mycobacterium tuberculosis, radiation biology and regeneration & repair.
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Organoids
Alveolus Lung-Organoids
We use feeder-free organoid cultures to expand alveolar type-2 epithelial cells for culture in Transwell or Lung-Chips. Organoids are additionally used to study (repair of) the alveolar micro-environment, alone or in co-culture with endothelial or structural cells.
Lung-Chips
Airway Lung-Chip
Our Airway Lung-Chip model is used to study how breathing biomechanics such as airflow and dynamic stretch affect primary airway epithelial cell biology .
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Lung-Chips
Alveolus Lung-Chip
We use a commercial Organs-on-Chips platform from Emulate Inc, to study how dynamic stretch affects alveolar epithelial cell biology.
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Lung-Chips
Alveolus Lung-Chip
An In-House Alveolus-on-Chip is used to study the interaction between 3D vasculature (vascularized hydrogel) and the epithelial compartment in the context of COPD.
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Lung-Chips
Fibrosis Lung-Chip
We use the Alveolus Lung-Chip as a starting point to develop a Fibrosis Lung-Chip model to study COVID-19-related lung damage.
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hiPSC
Alveolar type-2 cells
We use hiPSC to generate alveolar type-2 cells. We aim to improve the differentiation protocols and use the cells to study alveolar repair. We also use these cells to develop environmental and genetic models of alveolar damage.
hiPSC
Endothelial cells
We are using hiPSC-derived endothelial cells to investigate how a microenvironment shapes the lung microvascular endothelial cell phenotype​, and how these contribute to alveolar repair​.
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